Bio-Protocol, Available online: Oct 16,, 2024
Key features
• We developed a cost-effective method to assess carbohydrate distribution in small samples based on differential solubility and spectrophotometry.
• Efficient carbohydrate partitioning methods reduce time and effort, especially for large sample sets.
• Cost-effective carbohydrate analysis method reduces expenses, promotes accessibility, and encourages adoption in research and quality control.
• Analysis of small samples from microalgal and A. thaliana seedlings has wide applicability in scientific and technological research.
Abstract
Carbohydrates serve crucial functions in most living cells, encompassing structural and metabolic roles. Within the realms of plant and algal biology, carbohydrate biosynthesis and partitioning play pivotal roles in growth, development, stress physiology, and various practical applications. These applications span diverse fields, including the food and feed industry, bioenergetics (biofuels), and environmental management. However, existing methods for carbohydrate determination tend to be costly and time-intensive. In response to that, we propose a novel approach to assess carbohydrate partitioning from small samples. This method leverages the differential solubility of various fractions, including soluble sugars, starch, and structural polymers (such as cellulose). After fractionation, a straightforward spectrophotometric analysis allows for the quantification of sugars.
