Spanish journal of agricultural research, Vol. 18, Nº. 1, 2020, 2020
Highlights
To our knowledge, this is the first time that the presence of F graminearum s.s. is reported associated with triticale in Argentina.
Abstract
Aim of study: To report the occurrence of Fusarium graminearum sensu stricto (s.s.) on triticale grains from field samples i Argentina and the potential mycotoxin production for these isolates. Area of study. Buenos Aires province, Argentina.
Material and methods: A total of 40 samples from different crops (barley, rye, triticale, and wheat) showing Fusaium head blight symptoms were taken during 2017/2018 harvest season. Colonies with colour and mycelium similar to Fusarium were taken and wee morphologically and molecularly identified. The potential to produce deoxynivalenol, 15-acetyl deoxynivalenol and zearalenones was determined. Also, the Koch’s postulates were used to evaluate the pathogenic capacity of the F graminearum s.s. isolates in triticale Main results: Two Fusarium isolates were identified morphologically as F graminearum, which were confirmed molecularly.by PCR using the specific Fg16 F/R primers pair and by sequencing red and tri101 genes. The sequences obtained were compared with those available in the NCBI database using BLAST tools, showing 99-100% homology with those belonging to F graminearum s.5.
The results demonstrated that F graminearum s.s. isolates were pathogenic when triticale spikes were inoculated by spraying under greenhouse conditions.